Separation of therapeutic oligonucleotides using ion-pair reversed-phase chromatography based on fundamental separation science
نویسندگان
چکیده
منابع مشابه
Separation of polyprenyl phosphate oligomerhomologues by reversed-phase ion-pair high-performance liquid chromatography.
A simple and rapid method of reversed-phase ion-pair high-performance liquid chromatography (HPLC) with UV-detection on a common C(18) column in an isocratic mode with the addition of tetrabutylammonium phosphate for the separation of polyprenyl phosphate oligomerhomologues has been developed. The method was successfully applied to assay the composition of polyprenyl phosphates obtained from p...
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Synthetic DNAand RNA-based oligonucleotides are among many successful biotherapeutic drugs to treat many different kinds of illnesses. They are synthesized in a multistep process. Although coupling efficiencies are high, the overall yield of oligos decreases as the number of cycles increases, with failure in coupling with single (N-1) and double (N-2) deletions as the major impurities. To ensur...
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ژورنال
عنوان ژورنال: Journal of chromatography open
سال: 2023
ISSN: ['2772-3917']
DOI: https://doi.org/10.1016/j.jcoa.2023.100079